63. Wheat germ agglutinin induced tyrosine phosphorylation of human spermatozoa
نویسندگان
چکیده
منابع مشابه
Wheat Germ Agglutinin
Procedures for the isolation, purification, and crystallization of wheat germ agglutinin are described. The agglutinin was purified 184-fold to homogeneity from commercial wheat germ lipase. A molecular weight of 23,500 was estimated for the protein by means of sedimentation equilibrium and sodium dodecyl sulfate gel electrophoresis. The agghxtinin is a glycoprotein. Amino acid and carbohydrate...
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Wheat germ agglutinin (WGA) stimulated neutrophils to produce significant levels of luminol-dependent chemiluminescence (CL). Since WGA is known to bind N-acetylglucosamine (GlcNAc) oligomers and N-acetylneuraminic acid (NANA), we attempted to determine which binding property of WGA is essential for induction of CL. The succinylated form of WGA (SuWGA), which is no longer able to bind NANA, was...
متن کاملDrosophila cell fusion induced by wheat germ agglutinin.
The effects of wheat germ agglutinin on Drosophila embryonic cell lines growing on cover-glasses was examined by scanning electron microscopy. At low concentrations of the lectin (5-10 mug/ml), cells spread against the glass surface and fused to form syncytia. At high concentration, damage to the cell surface was evidenced as extensive membrane shrivelling and loss of surface microfilaments. Fu...
متن کاملFractionation of human T lymphocytes on wheat germ agglutinin-sepharose
T cells from human peripheral blood was purified by fractionation on columns charged with human immunoglobulin and rabbit anti-human immuno-globulin. When assayed with 125I- or fluorescein isothiocyanate-labeled wheat-germ agglutinin (WGA), a weakly binding and a strongly binding subpopulation could be distinguished. These T-cell subpopulations were fractionated on columns charged with WGA, con...
متن کاملTools - Based Search for Potential Human Wheat Germ Agglutinin - Immunoreactive
Following the initial observation about the exsistence of plant lectins-immunoreactive material in different rat and human tissues (D ebb age et al. 1993), we isolated a specific wheat germ agglutinin (WGA)-immunoreactive (IR) protein from human placenta by combination of ion-exchange and immunoaffinity chromatography. It was characterized as 66 kD glycoprotein exhibiting calcium-dependent, car...
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ژورنال
عنوان ژورنال: Reproduction, Fertility and Development
سال: 2003
ISSN: 1031-3613
DOI: 10.1071/srb03ab63